Structural insight into the calcium ion modulated interdomain electron transfer in cellobiose dehydrogenase

FEBS Lett. 2015 May 8;589(11):1194-9. doi: 10.1016/j.febslet.2015.03.029. Epub 2015 Apr 8.


Cellobiose dehydrogenase (CDH) from wood degrading fungi represents a subclass of oxidoreductases with unique properties. Consisting of two domains exhibiting interdomain electron transfer, this is the only known flavocytochrome involved in wood degradation. High resolution structures of the separated domains were solved, but the overall architecture of the intact protein and the exact interface of the two domains is unknown. Recently, it was shown that divalent cations modulate the activity of CDH and its pH optimum and a possible mechanism involving bridging of negative charges by calcium ions was proposed. Here we provide a structural explanation of this phenomenon confirming the interaction between negatively charged surface patches and calcium ions at the domain interface.

Keywords: Calcium effect; Cellobiose dehydrogenase; Electrostatic interaction; Flavocytochrome; Hydrogen/deuterium exchange; Interdomain electron transfer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / chemistry*
  • Carbohydrate Dehydrogenases / chemistry*
  • Electron Transport / physiology
  • Fungal Proteins / chemistry*
  • Protein Structure, Tertiary
  • Sordariales / enzymology*
  • Structure-Activity Relationship


  • Fungal Proteins
  • Carbohydrate Dehydrogenases
  • cellobiose-quinone oxidoreductase
  • Calcium