Abstract
A sensitive signal generation mechanism for gold nanoparticle growth by reducing gold ions with hydrogen peroxide is applied in a plasmonic aptasensor, achieving naked-eye detection of thrombin at the single-molecule level based on the specific interaction of aptamer-thrombin via an arrested rolling circle amplification to yield horseradish peroxidase (HRP)-mimicking DNAzymes as biocatalysts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aptamers, Nucleotide / chemistry
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Aptamers, Nucleotide / metabolism
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Biocatalysis
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DNA Replication*
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DNA, Catalytic / chemistry
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DNA, Catalytic / metabolism*
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DNA, Circular*
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Gold / chemistry*
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Gold / metabolism
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Horseradish Peroxidase / chemistry
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Horseradish Peroxidase / metabolism*
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Hydrogen Peroxide / chemistry
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Hydrogen Peroxide / metabolism
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Metal Nanoparticles / chemistry*
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Thrombin / analysis*
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Thrombin / metabolism
Substances
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Aptamers, Nucleotide
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DNA, Catalytic
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DNA, Circular
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thrombin aptamer
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Gold
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Hydrogen Peroxide
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Horseradish Peroxidase
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Thrombin