[Determination of the substrate specificity of Bpu101 restrictase with an unusual recognition segment]

S Kh Degtiarev; P A Zhilkin; G G Prikhod'ko; N I Rechkunova; V E Repin

[Determination of the substrate specificity of Bpu101 restrictase with an unusual recognition segment]

Mol Biol (Mosk). 1989 Jul-Aug;23(4):1051-6.

[Article in Russian]

Authors

S Kh Degtiarev, P A Zhilkin, G G Prikhod'ko, N I Rechkunova, V E Repin

PMID: 2586500

Abstract

A new enzyme Bpu10I was isolated from Bacillus pumilus. This enzyme is not an isoschizomer of any known restriction endonucleases. The search of possible recognition sequences was carried out in sequences ABCNiDEF (i = 0.6) on substrate DNA lambda CI857, T7, pBR322. The recognition sequence and cleavage sites of restriction endonuclease Bpu10I have been determined as CCTNAGC. GGANTCG

MeSH terms

Bacillus / enzymology*
Base Sequence
Deoxyribonucleases, Type II Site-Specific / metabolism*
Electrophoresis, Polyacrylamide Gel
Hydrolysis
Molecular Sequence Data
Substrate Specificity

Substances

endodeoxyribonuclease Bpu10I
Deoxyribonucleases, Type II Site-Specific