In Vivo Priming of Virus-Specific Cytotoxic T Lymphocytes With Synthetic Lipopeptide Vaccine

Nature. 1989 Nov 30;342(6249):561-4. doi: 10.1038/342561a0.

Abstract

Cytotoxic T lymphocytes (CTL) constitute an essential part of the immune response against viral infections. Such CTL recognize peptides derived from viral proteins together with major histocompatibility complex (MHC) class I molecules on the surface of infected cells, and usually require in vivo priming with infectious virus. Here we report that synthetic viral peptides covalently linked to tripalmitoyl-S-glycerylcysteinyl-seryl-serine (P3CSS) can efficiently prime influenza-virus-specific CTL in vivo. These lipopeptides are able to induce the same high-affinity CTL as does the infectious virus. Our data are not only relevant to vaccine development, but also have a bearing on basic immune processes leading to the transition of virgin T cells to activated effector cells in vivo, and to antigen presentation by MHC class I molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigen-Presenting Cells / immunology
  • Cytotoxicity, Immunologic*
  • H-2 Antigens / immunology
  • Lipoproteins / immunology*
  • Mice
  • Molecular Sequence Data
  • Peptide Fragments / immunology
  • T-Lymphocytes, Cytotoxic / immunology*
  • Vaccines / immunology*
  • Vaccines, Synthetic / immunology*
  • Viral Proteins / genetics
  • Viral Proteins / immunology
  • Viral Vaccines / immunology*

Substances

  • H-2 Antigens
  • Lipoproteins
  • Peptide Fragments
  • Vaccines
  • Vaccines, Synthetic
  • Viral Proteins
  • Viral Vaccines
  • N-palmitoyl-5,6-dipalmitoyl-S-glycerylcysteinyl-seryl-serine