Effects of non-equilibrium plasma in the treatment of ligature-induced peri-implantitis

J Clin Periodontol. 2015 May;42(5):478-87. doi: 10.1111/jcpe.12403.


Aim: To evaluate the effects of non-equilibrium plasma in the treatment of ligature-induced peri-implantitis in beagle dogs.

Materials and methods: Six beagles received 12 implants installed in the position of the fourth mandibular premolars. Ligature-induced peri-implantitis was initiated at 3 months post-implantation. When approximately 40% of the supporting bone was lost, the ligatures were removed. The implants were subjected to the muco-periosteal scaling and chlorhexidine irrigation with or without plasma irrigation. Three months later, clinical, radiographic and microbiological analyses were performed. Block biopsies were prepared for micro-CT and histomorphometric analysis. The primary outcome was the difference in bone healing of peri-implant sites, and the secondary outcomes included changes in clinical parameters (SBI, PD) and bacterial detection.

Results: At baseline, no significant differences were observed between the two groups. At 3 months post-treatment, the plasma group showed a significantly higher bone level than the control group (p < 0.05), a significantly decreased detection of bacteria (Porphyromonas gingivalis and Tannerella forsythia) (p < 0.05), and a significant improvement in clinical examination (p < 0.05).

Conclusions: Within the limits of this study, non-equilibrium plasma treatment as an adjunct to the conventional therapy is a feasible approach for the treatment of peri-implantitis.

Keywords: animal experiment; dental implants; infections; non-equilibrium plasma; peri-implantitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aggregatibacter actinomycetemcomitans / isolation & purification
  • Animals
  • Anti-Infective Agents, Local / therapeutic use
  • Bacteroides / isolation & purification
  • Biopsy / methods
  • Chlorhexidine / analogs & derivatives
  • Chlorhexidine / therapeutic use
  • Dental Implants
  • Disease Models, Animal
  • Dogs
  • Feasibility Studies
  • Peri-Implantitis / microbiology
  • Peri-Implantitis / pathology
  • Peri-Implantitis / therapy*
  • Periodontal Index
  • Periodontal Pocket / microbiology
  • Periodontal Pocket / pathology
  • Periodontal Pocket / therapy
  • Plasma Gases / therapeutic use*
  • Porphyromonas gingivalis / isolation & purification
  • Subgingival Curettage / methods
  • Therapeutic Irrigation / methods
  • Time Factors
  • Tomography, X-Ray Computed / methods
  • Tooth Socket / surgery
  • Wound Healing / physiology
  • X-Ray Microtomography / methods


  • Anti-Infective Agents, Local
  • Dental Implants
  • Plasma Gases
  • chlorhexidine gluconate
  • Chlorhexidine