Hepatoprotective effect of a polysaccharide from Crassostrea gigas on acute and chronic models of liver injury

Int J Biol Macromol. 2015:78:142-8. doi: 10.1016/j.ijbiomac.2015.03.056. Epub 2015 Apr 11.

Abstract

A water-soluble polysaccharide was isolated from Crassostrea gigas (CGPS-1) by hot-water extraction, isoelectric precipitation, hydrolysis and ultrafiltration without using ethanol. Based on the calibration with Dextran, CGPS-1 had a molecular weight of approximately 6.5×10(6) Da. CGPS-1 was analyzed using high-performance liquid chromatography, Fourier-transform infrared spectroscopy and (1)H and (13)C nuclear magnetic resonance spectroscopy. Based on the data, CGPS-1 was found to be a uniform glucose polymer. The protective effect of CGPS-1 on acute carbon tetrachloride- and chronic ethanol-induced liver injury was investigated in mice. Treatment with CGPS-1 decreased serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), and malondialdehyde (MDA) levels; increased superoxide dismutase (SOD) activity; and improved hepatic injury in both the acute and chronic models of liver injury in mice. These results suggest that CGPS-1 possesses potent hepatoprotective activity.

Keywords: Carbon tetrachloride; Crassostrea gigas; Ethanol; Liver injury; Polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbon-13 Magnetic Resonance Spectroscopy
  • Chemical and Drug Induced Liver Injury / drug therapy*
  • Chemical and Drug Induced Liver Injury / pathology*
  • Crassostrea / chemistry*
  • Disease Models, Animal
  • Female
  • Liver Function Tests
  • Male
  • Mice
  • Molecular Weight
  • Polysaccharides / chemistry*
  • Polysaccharides / pharmacology*
  • Protective Agents / chemistry*
  • Protective Agents / pharmacology*
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Polysaccharides
  • Protective Agents