A new quorum-sensing system (TprA/PhrA) for Streptococcus pneumoniae D39 that regulates a lantibiotic biosynthesis gene cluster

Mol Microbiol. 2015 Jul;97(2):229-43. doi: 10.1111/mmi.13029. Epub 2015 May 26.


The Phr peptides of the Bacillus species mediate quorum sensing, but their identification and function in other species of bacteria have not been determined. We have identified a Phr peptide quorum-sensing system (TprA/PhrA) that controls the expression of a lantibiotic gene cluster in the Gram-positive human pathogen, Streptococcus pneumoniae. Lantibiotics are highly modified peptides that are part of the bacteriocin family of antimicrobial peptides. We have characterized the basic mechanism for a Phr-peptide signaling system in S. pneumoniae and found that it induces the expression of the lantibiotic genes when pneumococcal cells are at high density in the presence of galactose, a main sugar of the human nasopharynx, a highly competitive microbial environment. Activity of the Phr peptide system is not seen when pneumococcal cells are grown with glucose, the preferred carbon source and the most prevalent sugar encountered by S. pneumoniae during invasive disease. Thus, the lantibiotic genes are expressed under the control of both cell density signals via the Phr peptide system and nutritional signals from the carbon source present, suggesting that quorum sensing and the lantibiotic machinery may help pneumococcal cells compete for space and resources during colonization of the nasopharynx.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / genetics
  • Bacillus / metabolism
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics
  • Bacteriocins / biosynthesis*
  • Bacteriocins / genetics*
  • Gene Expression Regulation, Bacterial*
  • Multigene Family*
  • Quorum Sensing / genetics
  • Quorum Sensing / physiology*
  • Streptococcus pneumoniae / genetics*
  • Streptococcus pneumoniae / metabolism*


  • Bacterial Proteins
  • Bacteriocins

Associated data

  • GEO/GSE68598