Novel insights into the behavioral analysis of mice subjected to the forced-swim test

Transl Psychiatry. 2015 Apr 14;5(4):e551. doi: 10.1038/tp.2015.44.


The forced-swim test (FST) is one of the most widely used rodent behavioral assays, in which the immobility of animals is used to assess the effectiveness of antidepressant drugs. However, the existing, and mostly arbitrary, criteria used for quantification could lead to biased results. Here we believe we uncovered new confounding factors, revealed new indices to interpret the behavior of mice and propose an unbiased means for quantification of the FST.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal*
  • Calbindin 1 / genetics
  • Confounding Factors, Epidemiologic
  • Depressive Disorder, Major*
  • Disease Models, Animal*
  • Female
  • Heterozygote
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Motor Activity*
  • Receptors, GABA-A / genetics
  • Swimming*


  • Calb1 protein, mouse
  • Calbindin 1
  • Gabra5 protein, mouse
  • Receptors, GABA-A