Fluorine-modified bisbenzimide derivative as a molecular probe for bimodal and simultaneous detection of DNAs by (19)F NMR and fluorescence

Chem Commun (Camb). 2015 May 25;51(42):8749-52. doi: 10.1039/c5cc01995h.

Abstract

3,5-Bis(trifluoromethyl)benzene modified bisbenzimide H 33258 was synthesized as a (19)F magnetic resonance-based DNA detection probe. The chemical shift and fluorescence of the probe were significantly changed by the addition of hairpin DNAs having an AATT sequence. The probe enables (19)F NMR/fluorescence bimodal detection of the model DNA double strands simultaneously.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bisbenzimidazole / analogs & derivatives*
  • Bisbenzimidazole / chemical synthesis
  • Bisbenzimidazole / chemistry*
  • DNA / analysis*
  • DNA Probes / chemical synthesis
  • DNA Probes / chemistry*
  • Fluorescence*
  • HeLa Cells
  • Humans
  • Magnetic Resonance Spectroscopy
  • Microscopy, Fluorescence
  • Molecular Structure
  • Toluene / analogs & derivatives*
  • Toluene / chemistry

Substances

  • DNA Probes
  • Toluene
  • DNA
  • Bisbenzimidazole