Multidrug resistance found in extended-spectrum beta-lactamase-producing Enterobacteriaceae from rural water reservoirs in Guantao, China

Hongna Zhang; Yufa Zhou; Shuyuan Guo; Weishan Chang

doi:10.3389/fmicb.2015.00267

Multidrug resistance found in extended-spectrum beta-lactamase-producing Enterobacteriaceae from rural water reservoirs in Guantao, China

Front Microbiol. 2015 Mar 31:6:267. doi: 10.3389/fmicb.2015.00267. eCollection 2015.

Authors

Hongna Zhang¹, Yufa Zhou², Shuyuan Guo¹, Weishan Chang¹

Affiliations

¹ College of Animal Science and Technology, Shandong Agricultural University , Taian, China.
² College of Animal Science and Technology, Shanxi Agricultural University , Taigu, China ; Animal Husbandry Bureau of Daiyue District , Taian, China.

Abstract

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae have been isolated from humans and animals across the world. However, data on prevalence of ESBL-producing Enterobacteriaceae from rural water reservoirs is limited. This study aimed to isolate and characterize ESBL-producing Enterobacteriaceae in rural water reservoirs in Guantao, China. ESBL-producing Enterobacteriaceae were found in 5 (16.7%) of 30 sampled rural water reservoirs. Sixty-six individual isolates expressing an ESBL phenotype were obtained in the present study. Species identification showed that 42 representatives of Escherichia coli, 17 Klebsiella pneumoniae, 4 Raoultella planticola, and 3 Enterobacter cloacae. Twenty isolates contained a single bla gene, including CTX-M (17 strains), TEM (2 strains), and SHV (1 strain). Forty-six isolates contained more than one type of beta-lactamase genes. ESBL-producing Enterobacteriaceae isolated in this study were all multidrug resistant. These findings indicated that the serious contamination of ESBL-producing Enterobacteriaceae in rural water reservoirs existed in Guantao, China.

Keywords: ESBL; Enterobacteriaceae; bla genes; multidrug resistance; rural water reservoirs.