Cell type-specific separation of subicular principal neurons during network activities

PLoS One. 2015 Apr 14;10(4):e0123636. doi: 10.1371/journal.pone.0123636. eCollection 2015.

Abstract

The hippocampal output structure, the subiculum, expresses two major memory relevant network rhythms, sharp wave ripple and gamma frequency oscillations. To this date, it remains unclear how the two distinct types of subicular principal cells, intrinsically bursting and regular spiking neurons, participate in these two network rhythms. Using concomitant local field potential and intracellular recordings in an in vitro mouse model that allows the investigation of both network rhythms, we found a cell type-specific segregation of principal neurons into participating intrinsically bursting and non-participating regular spiking cells. However, if regular spiking cells were kept at a more depolarized level, they did participate in a specific manner, suggesting a potential bimodal working model dependent on the level of excitation. Furthermore, intrinsically bursting and regular spiking cells exhibited divergent intrinsic membrane and synaptic properties in the active network. Thus, our results suggest a cell-type-specific segregation of principal cells into two separate groups during network activities, supporting the idea of two parallel streams of information processing within the subiculum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials
  • Animals
  • Female
  • Hippocampus / physiology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neurons / physiology*
  • Patch-Clamp Techniques
  • Synapses / physiology

Grants and funding

This study was supported by the DFG (GL 254/5-2), BMBF (BCCN II) and Einstein Foundation Berlin (A-2013-176) to TG, and Cluster Neurocure (Female PostDoc project) to TD. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.