Effects of Dietary Supplementation With Glutamate and Aspartate on Diquat-Induced Oxidative Stress in Piglets

PLoS One. 2015 Apr 15;10(4):e0122893. doi: 10.1371/journal.pone.0122893. eCollection 2015.


This study aimed to investigate the protective effects of dietary glutamate and aspartate supplementations on diquat-induced oxidative stress in piglets. Diquat injection significantly reduced growth performance, including body weight, average daily weight gain, and feed intake (P<0.05). Meanwhile, diquat administration induced oxidative stress evidenced by the decreased serum nitric oxide (NO) and elevated malondialdeyhde (MDA) concentration (P<0.05). Furthermore, diquat-induced oxidative stress disrupted intestinal absorption system and decreased serum threonine, serine, and glycine levels. Dietary supplementation with glutamate improved final body weight, antioxidant system, and expressions of amino acids transporters and enhanced serum glutamate concentration compared with diquat group (P<0.05). While aspartate failed to alleviate diquat-induced oxidative stress, growth depression, and dysfunction of nutrients absorption except for liver relative weight. In conclusion, dietary supplementation with glutamate confers beneficial effects on diquat-induced oxidative stress in piglets, while aspartate exhibits little effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / blood
  • Animals
  • Animals, Newborn
  • Antioxidants / metabolism
  • Aspartic Acid / administration & dosage
  • Aspartic Acid / pharmacology*
  • Body Weight / drug effects
  • Cationic Amino Acid Transporter 1 / genetics
  • Dietary Supplements*
  • Diquat / administration & dosage
  • Diquat / toxicity*
  • Excitatory Amino Acid Transporter 3 / genetics
  • Gene Expression / drug effects
  • Glutamic Acid / administration & dosage
  • Glutamic Acid / pharmacology*
  • Herbicides / administration & dosage
  • Herbicides / toxicity
  • Intestinal Mucosa / metabolism
  • Intestines / drug effects
  • Malondialdehyde / blood
  • Nitric Oxide / blood
  • Oxidative Stress / drug effects*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Superoxide Dismutase / blood
  • Swine
  • Weaning


  • Amino Acids
  • Antioxidants
  • Cationic Amino Acid Transporter 1
  • Excitatory Amino Acid Transporter 3
  • Herbicides
  • Aspartic Acid
  • Nitric Oxide
  • Glutamic Acid
  • Malondialdehyde
  • Diquat
  • Superoxide Dismutase

Grant support

The present work was supported by grants from the National Natural Science Foundation of China (Nos. 31330075, 31110103909, 31101729, 31402088, 31201813, and 31272463) and the Hunan Provincial Natural Science Foundation of China (Nos. 12JJ2014, 2013RS4065, and 12JJ2020). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.