Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate

Appl Biochem Biotechnol. 2015 Jun;176(3):875-91. doi: 10.1007/s12010-015-1617-z. Epub 2015 Apr 16.

Abstract

Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers. It is produced by algae and some species of Azotobacter and Pseudomonas. This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter. Results showed that alginate production increased with increasing DOT from 1 to 5 %. The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT. At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration. On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / chemistry*
  • Alginates / metabolism*
  • Azotobacter vinelandii / drug effects
  • Azotobacter vinelandii / metabolism
  • Azotobacter vinelandii / physiology
  • Biomass
  • Calcium / pharmacology
  • Culture Media / chemistry*
  • Dose-Response Relationship, Drug
  • Fermentation / drug effects
  • Glucuronic Acid / biosynthesis
  • Glucuronic Acid / chemistry
  • Glucuronic Acid / metabolism
  • Hexuronic Acids / chemistry
  • Hexuronic Acids / metabolism
  • Oxygen / chemistry*
  • Oxygen / pharmacology*
  • Stress, Physiological / drug effects*
  • Sucrose / pharmacology

Substances

  • Alginates
  • Culture Media
  • Hexuronic Acids
  • Sucrose
  • Glucuronic Acid
  • Oxygen
  • Calcium