DNA strand displacement reaction for programmable release of biomolecules

Chem Commun (Camb). 2015 May 14;51(39):8307-10. doi: 10.1039/c5cc01880c.

Abstract

Sample cleanup is a major processing step in many analytical assays. Here, we propose an approach to capture-and-release of analytes based on the DNA strand displacement reaction (SDR) and demonstrate its application to a fluoroimmunoassay on beads for a thyroid cancer biomarker, thyroglobulin. The SDR-based cleanup showed no interference from matrix molecules in serum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / chemistry
  • Antibodies / immunology
  • DNA / chemistry
  • Fluoresceins / chemistry
  • Fluoroimmunoassay
  • Humans
  • Serum Albumin, Bovine / chemistry
  • Thyroglobulin / blood*
  • Thyroglobulin / immunology

Substances

  • Antibodies
  • Fluoresceins
  • Serum Albumin, Bovine
  • 6-carboxyfluorescein
  • DNA
  • Thyroglobulin