Comparative Evaluation of Two PCR-Based Methods for Detection of Methicillin-Resistant Staphylococcus aureus (MRSA): Xpert MRSA Gen 3 and BD-Max MRSA XT

Margaux Lepainteur; Sandrine Delattre; Sophie Cozza; Christine Lawrence; Anne-Laure Roux; Martin Rottman

doi:10.1128/JCM.03679-14

Comparative Evaluation of Two PCR-Based Methods for Detection of Methicillin-Resistant Staphylococcus aureus (MRSA): Xpert MRSA Gen 3 and BD-Max MRSA XT

J Clin Microbiol. 2015 Jun;53(6):1955-8. doi: 10.1128/JCM.03679-14. Epub 2015 Apr 15.

Authors

Margaux Lepainteur¹, Sandrine Delattre², Sophie Cozza², Christine Lawrence¹, Anne-Laure Roux³, Martin Rottman⁴

Affiliations

¹ Infection Control Team, Hôpital Raymond Poincaré, AP-HP, Garches, France Microbiology Laboratory, Hôpital Raymond Poincaré, AP-HP, Garches, France.
² Microbiology Laboratory, Hôpital Raymond Poincaré, AP-HP, Garches, France.
³ Microbiology Laboratory, Hôpital Raymond Poincaré, AP-HP, Garches, France INSERM U1173, UFR Simone Veil, Versailles-Saint-Quentin University, Saint-Quentin en Yvelines, France.
⁴ Microbiology Laboratory, Hôpital Raymond Poincaré, AP-HP, Garches, France INSERM U1173, UFR Simone Veil, Versailles-Saint-Quentin University, Saint-Quentin en Yvelines, France martin.rottman@rpc.aphp.fr.

Abstract

We compared two walk-away molecular diagnostic assays, the GeneXpert MRSA Gen 3 assay and the BD-Max MRSA XT assay. A total of 119 prospective swabs and 36 culture-positive samples were tested. Xpert MRSA Gen 3 had sensitivity of 95.7% and specificity of 100% versus 87.5% and 97.1% for BD-Max. The difference in agreement with the enriched culture results was significantly in favor of the Xpert assay (P < 0.02, McNemar nonparametric text).

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Humans
Methicillin-Resistant Staphylococcus aureus / genetics*
Molecular Diagnostic Techniques / methods*
Nasal Cavity / microbiology
Polymerase Chain Reaction / methods*
Prospective Studies
Reproducibility of Results
Sensitivity and Specificity
Staphylococcal Infections / diagnosis*
Staphylococcal Infections / microbiology