Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes

Chem Commun (Camb). 2015 May 14;51(39):8319-22. doi: 10.1039/c5cc01613d.

Abstract

We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amides / chemistry
  • Coordination Complexes / chemistry*
  • Heterocyclic Compounds, 1-Ring / chemistry
  • Ligands
  • Luminescence
  • NADP / chemistry*
  • Terbium / chemistry*

Substances

  • Amides
  • Coordination Complexes
  • Heterocyclic Compounds, 1-Ring
  • Ligands
  • Terbium
  • NADP