Gateway-compatible tissue-specific vectors for plant transformation

BMC Res Notes. 2015 Mar 3:8:63. doi: 10.1186/s13104-015-1010-6.

Abstract

Background: Understanding regulation of developmental events has increasingly required the use of tissue-specific expression of diverse genes affecting plant growth and environmental responses.

Findings: To allow for cloning of presumptive promoters with tissue-specific activities, we created two plant expression vectors with multiple cloning sites upstream of a Gateway cassette for expression of either untagged or YFP-tagged genes of interest. For fast and easy tissue-specific expression of desired genes, we further developed an initial set of Gateway-compatible tissue-specific gene expression vectors that allow for the expression of YFP-tagged or untagged proteins driven by the ALCOHOL DEHYDROGENASE1, CHLOROPHYLL A/B BINDING PROTEIN 1, COBRA LIKE1, EXPANSIN7, LATERAL ORGAN BOUNDARIES-DOMAIN 16, SCARECROW, UBIQUITIN10, and WOODEN LEG upstream regulatory regions.

Conclusions: These vectors provide an invaluable resource to the plant community, allowing for rapid generation of a variety of tissue-specific expression constructs.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Agrobacterium tumefaciens / genetics
  • Alcohol Dehydrogenase / genetics
  • Arabidopsis / genetics*
  • Arabidopsis Proteins / genetics
  • Bacterial Proteins / genetics
  • Base Sequence
  • Chlorophyll Binding Proteins / genetics
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Gene Transfer Techniques*
  • Genes, Reporter
  • Genetic Vectors / chemistry*
  • Genetic Vectors / metabolism
  • Luminescent Proteins / genetics
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Transcription Factors / genetics
  • Transformation, Genetic*
  • Ubiquitin / genetics

Substances

  • Arabidopsis Proteins
  • Bacterial Proteins
  • Chlorophyll Binding Proteins
  • EXPANSIN A7 protein, Arabidopsis
  • LBD16 protein, Arabidopsis
  • Luminescent Proteins
  • SCR protein, Arabidopsis
  • Transcription Factors
  • Ubiquitin
  • yellow fluorescent protein, Bacteria
  • Alcohol Dehydrogenase