Chemoenzymatic Synthesis of (36)S Isotopologues of Methionine and S-Adenosyl-L-methionine

J Org Chem. 2015 May 15;80(10):5344-7. doi: 10.1021/acs.joc.5b00608. Epub 2015 Apr 23.


Substrates containing isotope labels at specific atoms are required for transition-state analysis based on the measurement of multiple kinetic isotope effects.(36)S-labeled l-methionine and S-adenosyl-l-methionine were synthesized from elemental sulfur using a chemoenzymatic approach with >98% (36)S enrichment. This method provides access to previously inaccessible sulfur isotope-labeled substrates for sulfur kinetic isotope effect studies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Isotope Labeling / methods*
  • Kinetics
  • Methionine / chemical synthesis*
  • Methionine / chemistry
  • Molecular Structure
  • S-Adenosylmethionine / chemical synthesis*
  • S-Adenosylmethionine / chemistry
  • Sulfur Radioisotopes / chemistry*


  • Sulfur Radioisotopes
  • S-Adenosylmethionine
  • Methionine