Lumican deficiency results in cardiomyocyte hypertrophy with altered collagen assembly

J Mol Cell Cardiol. 2015 Jul;84:70-80. doi: 10.1016/j.yjmcc.2015.04.007. Epub 2015 Apr 15.

Abstract

The ability of the heart to adapt to increased stress is dependent on the modification of its extracellular matrix (ECM) architecture that is established during postnatal development as cardiomyocytes differentiate, a process that is poorly understood. We hypothesized that the small leucine-rich proteoglycan (SLRP) lumican (LUM), which binds collagen and facilitates collagen assembly in other tissues, may play a critical role in establishing the postnatal murine myocardial ECM. Although previous studies suggest that LUM deficient mice (lum(-/-)) exhibit skin anomalies consistent with Ehlers-Danlos syndrome, lum(-/-) hearts have not been evaluated. These studies show that LUM was immunolocalized to non-cardiomyocytes of the cardiac ventricles and its expression increased throughout development. Lumican deficiency resulted in significant (50%) perinatal death and further examination of the lum(-/-) neonatal hearts revealed an increase in myocardial tissue without a significant increase in cell proliferation. However cardiomyocytes from surviving postnatal day 0 (P0), 1 month (1 mo) and adult (4 mo) lum(-/-) hearts were significantly larger than their wild type (WT) littermates. Immunohistochemistry revealed that the increased cardiomyocyte size in the lum(-/-) hearts correlated with alteration of the cardiomyocyte pericellular ECM components collagenα1(I) and the class I SLRP decorin (DCN). Western blot analysis demonstrated that the ratio of glycosaminoglycan (GAG) decorated DCN to core DCN was reduced in P0 and 1 mo lum(-/-) hearts. There was also a reduction in the β and γ forms of collagenα1(I) in lum(-/-) hearts. While the total insoluble collagen content was significantly reduced, the fibril size was increased in lum(-/-) hearts, indicating that LUM may play a role in collagen fiber stability and lateral fibril assembly. These results suggest that LUM controls cardiomyocyte growth by regulating the pericellular ECM and also indicates that LUM may coordinate multiple factors of collagen assembly in the murine heart. Further investigation into the role of LUM may yield novel therapeutic targets and/or biomarkers for patients with cardiovascular disease.

Keywords: Cardiomyocyte; Collagen; Decorin; Lumican; Myocardium; SLRP.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Cell Size
  • Chondroitin Sulfate Proteoglycans / deficiency*
  • Chondroitin Sulfate Proteoglycans / metabolism
  • Collagen / metabolism*
  • Collagen / ultrastructure
  • Decorin / metabolism
  • Embryonic Development
  • Fetus / metabolism
  • Glycosaminoglycans / metabolism
  • Heart Ventricles / metabolism
  • Hypertrophy
  • Keratan Sulfate / deficiency*
  • Keratan Sulfate / metabolism
  • Lumican
  • Mice, Inbred C57BL
  • Models, Biological
  • Molecular Weight
  • Myocardium / metabolism
  • Myocytes, Cardiac / metabolism*
  • Myocytes, Cardiac / pathology*
  • Myocytes, Cardiac / ultrastructure
  • Protein Isoforms / metabolism
  • Solubility

Substances

  • Chondroitin Sulfate Proteoglycans
  • Decorin
  • Glycosaminoglycans
  • Lum protein, mouse
  • Lumican
  • Protein Isoforms
  • Collagen
  • Keratan Sulfate