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. 2015 Apr 16;15(1):48.
doi: 10.1093/jisesa/iev032. Print 2015.

The Complete Mitochondrial Genome of Rondotia Menciana (Lepidoptera: Bombycidae)

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Free PMC article

The Complete Mitochondrial Genome of Rondotia Menciana (Lepidoptera: Bombycidae)

Weiqing Kong et al. J Insect Sci. .
Free PMC article

Abstract

The mulberry white caterpillar, Rondotia menciana Moore (Lepidoptera: Bombycidae) is a species with closest relationship with Bombyx mori and Bombyx mandarina, and the genetic information of R. menciana is important for understanding the diversity of the Bombycidae. In this study, the mitochondrial genome (mitogenome) of R. menciana was amplified by polymerase chain reaction and sequenced. The mitogenome of R. menciana was determined to be 15,301 bp, including 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, 22 transfer RNA genes, and an AT-rich region. The A+T content (78.87%) was lower than that observed for other Bombycidae insects. All PCGs were initiated by ATN codons and terminated with the canonical stop codons, except for coxII, which was terminated by a single T. All the tRNA genes displayed a typical clover-leaf structure of mitochondrial tRNA. The length of AT-rich region (360 bp) of R. menciana mitogenome is shorter than that of other Bombycidae species. Phylogenetic analysis showed that the R. menciana was clustered on one branch with B. mori and B. mandarina from Bombycidae.

Keywords: Bombycidae; diversity; mitogenome; phylogeny.

Figures

Fig. 1.
Fig. 1.
Map of the mitogenome of R. menciana. The direction of all PCGs is designed by the underlined arrows. The transfer RNA genes are designated by single-letter amino acids codes. L1, L2, S1, and S2 denote trnL(UUR), trnL(CUN), trnS(AGN), and trnS(UCN), respectively.
Fig. 2.
Fig. 2.
Putative secondary cloverleaf structures for the tRNA genes of the R. menciana mitogenome with mismatch bases. The blue point and red point indicate Watson–Crick base pairing A-U and G-C, respectively, and the blank indicate the mismatch bases. Six mismatches (four U-U, one A-G, and one U-G) lies in five tRNA genes (two in amino acid acceptor stems, three in anticodon stems and one in pseudouridine) (TΨC).
Fig. 3.
Fig. 3.
Alignments of the A + T-rich region in Bombycidae. The thread underlined and thick overlined indicate the tandom repeat in R. menciana, and B. mori and B. mandarina, respectively.
Fig. 4.
Fig. 4.
Phylogenetic analysis inferred from the concatenated nucleotides sequences of 13 PCGs of mitogenome using Mega 5.05 software and ML method. D. melanogaster and T. castaneum were used as outgroups. The numbers above the branches specify bootstrap percentages (1,000 replicates). *This study.

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References

    1. Avise J. C. 1987. Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. Annu. Rev. Ecol. Syst. 18: 489–522.
    1. Ballard J.W.O. 2000. Comparative genomics of mitochondrial DNA in Drosophila simulans. J. Mol. Evol. 51: 64–75. - PubMed
    1. Ballard J.W.O., Rand D. M. 2005. The population biology of mitochondrial DNA and its phylogenetic implications. Annu. Rev. Ecol. Evol. Syst. 36: 621–642.
    1. Benson G. 1999. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res. 27: 573–580. - PMC - PubMed
    1. Boore J. L. 1999. Animal mitochondrial genomes. Nucleic Acids Res. 27: 1767–1780. - PMC - PubMed

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