Viability and functional assessment of murine pancreatic islets after transportation between Korea and Japan

Transplant Proc. 2015 Apr;47(3):738-41. doi: 10.1016/j.transproceed.2014.12.031.

Abstract

Background: Organ donor scarcity remains a restricting factor for pancreatic islet transplantation. To date, limited information is available on the impact of long-distance transportation on transplantable pancreatic islets. The objective of this study was to assess the effects of transportation on the viability and function of murine pancreatic islet cells.

Methods: The isolated murine pancreatic islets were transported from Japan to Korea with the use of commercial modes of transportation: subway and commercial airplane. After transportation, the islets were assessed by performing a viability assay and by evaluating the islets' insulin secretion in response to glucose stimulation. A comparative study was performed for evaluating the insulin secretory responses of transported and control islets (not transported).

Results: There was no evidence of contamination in the transported pancreatic islets. No significant differences were observed in the viability and functionality of the transported and control islet cells.

Conclusions: These findings show the feasibility of pancreatic islet transportation from Japan to Korea. Our data could be used not only for the inter-Asian but also for global advancement of animal and human islet transportation methods and transplantation research.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Feasibility Studies
  • Humans
  • International Cooperation
  • Islets of Langerhans / physiology*
  • Islets of Langerhans Transplantation*
  • Japan
  • Mice
  • Mice, Inbred C57BL
  • Republic of Korea
  • Tissue and Organ Procurement / methods*
  • Transportation*