Secondary structure and (1)H, (13)C, (15)N resonance assignments of the endosomal sorting protein sorting nexin 3

Biomol NMR Assign. 2015 Oct;9(2):355-8. doi: 10.1007/s12104-015-9609-z. Epub 2015 Apr 19.

Abstract

Sorting nexin 3 (SNX3) belongs to a sub-family of sorting nexins that primarily contain a single Phox homology domain capable of binding phosphoinositides and membranes. We report the complete (1)H, (13)C and (15)N resonance assignments of the full-length human SNX3 protein and identification of its secondary structure elements, revealing a canonical fold and unstructured termini.

Keywords: Endosome; NMR; PX domain; Phosphatidylinositol 3-phosphate; Phosphoinositide recognition; SNX3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carbon-13 Magnetic Resonance Spectroscopy*
  • Endosomes / metabolism*
  • Humans
  • Molecular Sequence Data
  • Nitrogen Isotopes
  • Protein Structure, Secondary
  • Proton Magnetic Resonance Spectroscopy*
  • Sorting Nexins / chemistry*

Substances

  • Nitrogen Isotopes
  • SNX3 protein, human
  • Sorting Nexins