A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy

Nat Methods. 2015 Jun;12(6):515-8. doi: 10.1038/nmeth.3362. Epub 2015 Apr 20.


Fluorescence nanoscopy has revolutionized our ability to visualize biological structures not resolvable by conventional microscopy. However, photodamage induced by intense light exposure has limited its use in live specimens. Here we describe Kohinoor, a fast-switching, positively photoswitchable fluorescent protein, and show that it has high photostability over many switching repeats. With Kohinoor, we achieved super-resolution imaging of live HeLa cells using biocompatible, ultralow laser intensity (0.004 J/cm(2)) in reversible saturable optical fluorescence transition (RESOLFT) nanoscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • HeLa Cells
  • Humans
  • Lasers*
  • Luminescent Proteins / chemistry*
  • Molecular Sequence Data
  • Nanotechnology / instrumentation*
  • Nanotechnology / methods*


  • Luminescent Proteins

Associated data

  • GENBANK/AB935555