[Genetic characterisation of Powassan virus (POWV) isolated from Haemophysalis longicornis ticks in Primorye and two strains of Tick-borne encephalitis virus (TBEV) (Flaviviridae, Flavivirus): Alma-Arasan virus (AAV) isolated from Ixodes persulcatus ticks in Kazakhstan and Malyshevo virus isolated from Aedes vexans nipponii mosquitoes in Khabarovsk kray]

Vopr Virusol. 2014;59(5):18-22.
[Article in Russian]

Abstract

The complete genomes of the three tick-borne flaviviruses (genus Flavivirus, fam. Bunyaviridae) were sequenced: Povassan virus (POWV, strain LEIV-3070Prm, isolated from Haemophysalis logicornis in Primorsky Krai, Russia in 1977), Alma-Arasan virus (AAV, strain LEIV-1380Kaz, isolated from Ixodes persulcatus ticks in Kazakhstan in 1977) and Malyshevo virus (isolated from a pool of Aedes vexans nipponii mosquitoes, in the Khabarovsk Krai, Russia in 1978). It is shown that AAV and Malyshevo virus are the strains of Tick-borne encephalitis virus (TBEV) and belong to Sibirian and Far-Eastern genotypes, respectively (GenBank ID: AAV KJ744033; strain Malyshevo KJ744034). Phylogenetically AAV is closest related (94,6% nt and 98,3% aa identity) to TBEV strains, isolated in Sibiria (Vasilchenko, Aino, Chita-653, Irkutsk-12). Malyshevo virus is closest related (96,4% nt and 98,3% nt identity) to strains of TBEV, isolated in Far Eastern part of Russia (1230, Spassk-72, Primorye-89). POWV LEIV-3070Prm has 99.7% identity with the prototype strain POWV LB, isolated in Canada and 99.5% of isolates with Far-Eastern strains of POWV (Spassk-9 and Nadezdinsk-1991).

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / virology*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Flaviviridae / classification
  • Flaviviridae / genetics*
  • Flaviviridae / isolation & purification
  • Flaviviridae Infections / transmission
  • Flaviviridae Infections / veterinary*
  • Flaviviridae Infections / virology
  • Genome, Viral*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Kazakhstan
  • Mice
  • Molecular Sequence Data
  • Phylogeny*
  • Sequence Homology, Nucleic Acid
  • Siberia
  • Ticks / virology*
  • Viral Proteins / genetics*

Substances

  • Viral Proteins

Associated data

  • GENBANK/KJ744033
  • GENBANK/KJ744034