Experimental Curative Fluorescence-guided Surgery of Highly Invasive Glioblastoma Multiforme Selectively Labeled With a Killer-reporter Adenovirus

Mol Ther. 2015 Jul;23(7):1182-1188. doi: 10.1038/mt.2015.63. Epub 2015 Apr 21.

Abstract

Fluorescence-guided surgery (FGS) of cancer is an area of intense current interest. However, although benefits have been demonstrated with FGS, curative strategies need to be developed. Glioblastoma multiforme (GBM) is one of the most invasive of cancers and is not totally resectable using standard bright-light surgery (BLS) or current FGS strategies. We report here a curative strategy for FGS of GBM. In this study, telomerase-dependent adenovirus OBP-401 infection brightly and selectively labeled GBM with green fluorescent protein (GFP) for FGS in orthotopic nude mouse models. OBP-401-based FGS enabled curative resection of GBM without recurrence for at least 150 days, compared to less than 30 days with BLS.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenoviridae
  • Animals
  • Cell Line, Tumor
  • Disease Models, Animal
  • Disease-Free Survival
  • Fluorescence*
  • Glioblastoma / pathology
  • Glioblastoma / surgery*
  • Green Fluorescent Proteins / chemistry
  • Green Fluorescent Proteins / therapeutic use*
  • Humans
  • Mice
  • Mice, Nude
  • Neoplasm Recurrence, Local / pathology
  • Neoplasm Recurrence, Local / surgery*
  • Optical Imaging

Substances

  • Green Fluorescent Proteins