Annona muricata leaves accelerate wound healing in rats via involvement of Hsp70 and antioxidant defence

Int J Surg. 2015 Jun;18:110-7. doi: 10.1016/j.ijsu.2015.03.026. Epub 2015 Apr 18.


Introduction: Annona muricata, a member of the Annonaceae family, is commonly known as soursop and graviola. The leaves of this tropical fruit tree are widely used in folk medicine against skin diseases and abscesses, however there is no scientific evidence justifying the use of A. muricata leaves. The aim of the present study is to evaluate the wound healing potential of ethyl acetate extract of A. muricata leaves (EEAM) towards excisional wound models in rats.

Methods: Sprague Dawley rats (24) were randomly divided into four groups, viz. (A) vehicle control, (B) low dose of EEAM (5% w/w), (C) high dose of EEAM (10% w/w) and (D) positive control with excisional wound created on the neck area. Wounds were topically dressed twice a day for 15 days. On the 15th day, animals were sacrificed and then processed for immunohistochemical and histological evaluations, including Hematoxylin & Eosin and Masson Trichrome stainings. The activity of antioxidants, namely catalase, glutathione peroxidase and superoxide dismutase, and malondialdehyde (MDA) was measured in wound tissue homogenate.

Results: Macroscopic and microscopic analysis of wounds demonstrated a significant wound healing activity shown by EEAM at two doses. Treatment of wounds with ointment containing EEAM caused significant surge in antioxidants activities and decrease in the MDA level of wound tissues compared with vehicle control. The immunohistochemical evaluation revealed conspicuous up-regulation of Hsp70 in treated wounds with EEAM, suggesting the anti-inflammatory effect of EEAM.

Conclusion: EEAM exhibited a promising wound healing potential towards excisional wound models in rats.

Keywords: Annona muricata; Annonaceae; Antioxidant defence; Hsp70; Inflammatory cells; Wound healing.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Annona*
  • Antioxidants / pharmacology*
  • Catalase / metabolism
  • HSP70 Heat-Shock Proteins / biosynthesis
  • Male
  • Malondialdehyde / metabolism
  • Phytotherapy / methods*
  • Plant Extracts / pharmacology
  • Plant Leaves
  • Rats, Sprague-Dawley
  • Skin / injuries*
  • Skin / metabolism
  • Superoxide Dismutase / metabolism
  • Up-Regulation / drug effects
  • Wound Healing / drug effects*
  • Wound Healing / physiology


  • Antioxidants
  • HSP70 Heat-Shock Proteins
  • Plant Extracts
  • Malondialdehyde
  • Catalase
  • Superoxide Dismutase