CD200+ and CD200- macrophages accumulated in ischemic lesions of rat brain: the two populations cannot be classified as either M1 or M2 macrophages

Shirabe Matsumoto; Junya Tanaka; Hajime Yano; Hisaaki Takahashi; Kana Sugimoto; Shiro Ohue; Akihiro Inoue; Hitomi Aono; Akari Kusakawa; Hideaki Watanabe; Yoshiaki Kumon; Takanori Ohnishi

doi:10.1016/j.jneuroim.2015.03.013

CD200+ and CD200- macrophages accumulated in ischemic lesions of rat brain: the two populations cannot be classified as either M1 or M2 macrophages

J Neuroimmunol. 2015 May 15:282:7-20. doi: 10.1016/j.jneuroim.2015.03.013. Epub 2015 Mar 14.

Authors

Affiliations

¹ Department of Neurosurgery, Graduate School of Medicine, Ehime University, Japan.
² Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Japan. Electronic address: jtanaka@m.ehime-u.ac.jp.
³ Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Japan.
⁴ Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Japan; Center for Advanced Research and Education, Asahikawa Medical University, Japan.
⁵ Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Japan; Department of Legal Medicine, Graduate School of Medicine/Faculty of Medicine, Osaka University, Japan.
⁶ Department of Regeneration of Community Medicine, Graduate School of Medicine, Ehime University, Japan.

PMID: 25903723
DOI: 10.1016/j.jneuroim.2015.03.013

Abstract

Two types of macrophages in lesion core of rat stroke model were identified according to NG2 chondroitin sulfate proteoglycan (NG2) and CD200 expression. NG2(+) macrophages were CD200(-), and vice versa. NG2(-) macrophages expressed two splice variants of CD200 that are CD200L and CD200S. CD200(+) macrophages expressed CD8, CD68, CD163, CCL2, inducible nitric oxide synthase, interleukin-1β, Toll-like receptor 4 and transforming growth factor β, whilst NG2(+) cells expressed a costimulatory factor CD86. Both cell types expressed insulin-like growth factor 1 and CD200R. These results demonstrate that the two macrophage types cannot be classified as either M1 or M2.

Keywords: Alternative; CD68; CD86; Ki67; NG2; TGFβ; TLR4; iNOS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens / metabolism
Antigens, CD / genetics
Antigens, CD / metabolism*
Bone Marrow Transplantation
Brain / metabolism
Brain / pathology*
Calcium-Binding Proteins / metabolism
Cell Count
Cytokines / genetics
Cytokines / metabolism
Disease Models, Animal
Gene Expression Regulation / physiology*
Infarction, Middle Cerebral Artery / pathology*
Infarction, Middle Cerebral Artery / surgery
Insulin-Like Growth Factor I / metabolism
Ki-67 Antigen / metabolism
Macrophages / classification*
Macrophages / metabolism*
Male
Microfilament Proteins / metabolism
Nitric Oxide Synthase Type II / metabolism
Proteoglycans / metabolism
Rats
Rats, Sprague-Dawley
Rats, Transgenic
Rats, Wistar
Toll-Like Receptor 4 / metabolism

Substances

Aif1 protein, rat
Antigens
Antigens, CD
Calcium-Binding Proteins
Cytokines
Ki-67 Antigen
Microfilament Proteins
Proteoglycans
Tlr4 protein, rat
Toll-Like Receptor 4
chondroitin sulfate proteoglycan 4
insulin-like growth factor-1, rat
Insulin-Like Growth Factor I
Nitric Oxide Synthase Type II
antigens, CD200