Comparison of nasopharyngeal aspirate with flocked swab for PCR-detection of respiratory viruses in children

APMIS. 2015 Jun;123(6):473-7. doi: 10.1111/apm.12375. Epub 2015 Apr 23.


Fast- and high-throughput molecular workflows require sample matrices to be suitable for automation. Respiratory swabs are better suited for this purpose compared to the more viscous nasopharyngeal aspirates. Samples collected by nasopharyngeal aspiration and nasopharyngeal flocked swab from 81 children were compared for detection and recovery of respiratory viruses. Using real-time RT-PCR, no statistically significant differences in virus detection between the two sample types were found, supporting the use of flocked swabs in children aged one month to two years.

Keywords: Aspirate; PCR; flocked swab; respiratory viruses.

Publication types

  • Comparative Study

MeSH terms

  • Child, Preschool
  • Humans
  • Infant
  • Nasopharynx / virology*
  • Real-Time Polymerase Chain Reaction / methods*
  • Respiratory Syncytial Viruses / isolation & purification*
  • Respiratory Tract Infections / diagnosis*
  • Respiratory Tract Infections / virology*
  • Sensitivity and Specificity
  • Specimen Handling
  • Viral Load