[Cytochrome P-450 in proteoliposomes: oligomeric structure of the LM2 isoform]

Biokhimiia. 1989 Sep;54(9):1445-56.
[Article in Russian]

Abstract

Crosslinking of protein molecules with bifunctional reagents and subsequent electrophoresis of the modified proteins revealed the presence of cytochrome P-450 LM 2 oligomers in proteoliposome membranes obtained in different ways and differing in their phospholipid composition. Data from a comparative analysis of cytochrome P-450 oligomeric structures in solution and in membrane are suggestive of the hexameric organization of cytochrome P-450 LM 2 within proteoliposome membranes.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Bacteriorhodopsins / metabolism
  • Chromatography, Gel
  • Cross-Linking Reagents
  • Cytochrome P-450 Enzyme System / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Isoenzymes / metabolism*
  • Liposomes / metabolism*
  • Microscopy, Electron
  • Microsomes, Liver / enzymology
  • Protein Conformation
  • Proteolipids / metabolism*
  • Rabbits

Substances

  • Cross-Linking Reagents
  • Isoenzymes
  • Liposomes
  • Proteolipids
  • proteoliposomes
  • Bacteriorhodopsins
  • Cytochrome P-450 Enzyme System