Induction of c-Jun by air particulate matter (PM₁₀) of Mexico city: Participation of polycyclic aromatic hydrocarbons

Environ Pollut. 2015 Aug:203:175-182. doi: 10.1016/j.envpol.2015.03.051. Epub 2015 Apr 20.


The carcinogenic potential of urban particulate matter (PM) has been partly attributed to polycyclic aromatic hydrocarbons (PAHs) content, which activates the aryl hydrocarbon receptor (AhR). Here we report the effect of PM with an aerodynamic size of 10 μm (PM10) on the induction of AhR pathway in A549 cells, evaluating its downstream targets CYP1B1, IL-6, IL-8 and c-Jun. Significant increases in CYP1B1 protein and enzyme activity; IL-6 and IL-8 secretion and c-Jun protein were found in response to PM10. The formation of PAH-DNA adducts was also detected. The involvement of AhR pathway was confirmed with Resveratrol as AhR antagonist, which reversed CYP1B1 and c-Jun induction. Nevertheless, in IL-6 and IL-8 secretion, the Resveratrol was ineffective, suggesting an effect independent of this pathway. Considering the role of c-Jun in oncogenesis, its induction by PM may be contributing to its carcinogenic potential through induction of AhR pathway by PAHs present in PM10.

Keywords: Particulate matter; aryl hydrocarbon receptor; cJun; carcinogenic potential; polycyclic aromatic hydrocarbons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Air Pollutants / toxicity*
  • Cell Line, Tumor
  • Cities
  • Cytochrome P-450 CYP1B1 / metabolism
  • DNA Adducts / metabolism
  • Humans
  • Interleukin-6 / metabolism
  • Interleukin-8 / metabolism
  • Mexico
  • Particulate Matter / toxicity*
  • Polycyclic Aromatic Hydrocarbons / metabolism
  • Polycyclic Aromatic Hydrocarbons / toxicity*
  • Proto-Oncogene Proteins c-jun / metabolism*
  • Receptors, Aryl Hydrocarbon / antagonists & inhibitors
  • Receptors, Aryl Hydrocarbon / metabolism
  • Resveratrol
  • Stilbenes / pharmacology


  • Air Pollutants
  • DNA Adducts
  • IL6 protein, human
  • Interleukin-6
  • Interleukin-8
  • Particulate Matter
  • Polycyclic Aromatic Hydrocarbons
  • Proto-Oncogene Proteins c-jun
  • Receptors, Aryl Hydrocarbon
  • Stilbenes
  • polycyclic aromatic hydrocarbons-DNA adduct
  • CYP1B1 protein, human
  • Cytochrome P-450 CYP1B1
  • Resveratrol