Structure of the Drosophila BicaudalD protein and its role in localizing the posterior determinant nanos

Cell. 1989 Dec 1;59(5):881-92. doi: 10.1016/0092-8674(89)90611-9.

Abstract

Mutations in the BicaudalD (BicD) gene lead to a global reorganization of the Drosophila body pattern such that the head, thoracic, and anterior abdominal segments are replaced by posterior abdominal segments and terminalia. We first provide evidence that the primary cause of this phenotype is the inhibition of two anterior factors, bicoid and hunchback, by mislocalized activity of the posterior determinant nanos. We then describe the isolation of the BicD gene and show that it encodes a coiled-coil protein similar to the carboxy-terminal portion of the myosin heavy chain. Finally, we find that BicD protein is uniformly distributed throughout wild-type oocytes but is concentrated at the anterior pole of BicD mutant oocytes together with ectopic nanos activity. Taken together, these results suggest that BicD encodes a cytoskeleton-like protein involved in transporting or anchoring the nanos morphogen within the oocyte cytoplasm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Drosophila / anatomy & histology
  • Drosophila / embryology
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Embryo, Nonmammalian
  • Female
  • Genes*
  • Insect Hormones / genetics*
  • Insect Hormones / metabolism
  • Molecular Sequence Data
  • Mutation*
  • Ovary / metabolism
  • Phenotype

Substances

  • BicD protein, Drosophila
  • Drosophila Proteins
  • Insect Hormones