Mechanical and biological properties of the micro-/nano-grain functionally graded hydroxyapatite bioceramics for bone tissue engineering

J Mech Behav Biomed Mater. 2015 Aug:48:1-11. doi: 10.1016/j.jmbbm.2015.04.002. Epub 2015 Apr 8.

Abstract

Functionally graded materials (FGM) open the promising approach for bone tissue repair. In this study, a novel functionally graded hydroxyapatite (HA) bioceramic with micrograin and nanograin structure was fabricated. Its mechanical properties were tailored by composition of micrograin and nanograin. The dynamic mechanical analysis (DMA) indicated that the graded HA ceramics had similar mechanical property compared to natural bones. Their cytocompatibility was evaluated via fluorescent microscopy and MTT colorimetric assay. The viability and proliferation of rabbit bone marrow mesenchymal stem cells (BMSCs) on ceramics indicated that this functionally graded HA ceramic had better cytocompatibility than conventional HA ceramic. This study demonstrated that functionally graded HA ceramics create suitable structures to satisfy both the mechanical and biological requirements of bone tissues.

Keywords: Biological property; Bone tissue engineering; Functionally graded materials; Mechanical property; Two-step sintering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Bone and Bones*
  • Cell Proliferation
  • Ceramics / chemistry*
  • Durapatite / chemistry*
  • Materials Testing
  • Mesenchymal Stem Cells / cytology
  • Rabbits
  • Tissue Engineering / methods*

Substances

  • Biocompatible Materials
  • Durapatite