Vetiver oil (Java) attenuates cisplatin-induced oxidative stress, nephrotoxicity and myelosuppression in Swiss albino mice

Food Chem Toxicol. 2015 Jul:81:120-128. doi: 10.1016/j.fct.2015.04.018. Epub 2015 Apr 21.


Clinical efficacy of the widely used anticancer drug cisplatin is limited due to its adverse side effects in normal tissues mediated by oxidative stress. This study was aimed to investigate the protective effects of vetiver acetate oil, Java (VO) against cisplatin-induced toxicity in Swiss albino mice. The ameliorating potential was evaluated by orally priming the animals with VO at doses 5, 10 or 20 mg/kg bw for 7 days prior to cisplatin treatment. Acute toxicity in mice was induced by injecting cisplatin (3 mg/kg bw) intraperitoneally for 5 consecutive days. Significant attenuation of renal toxicity was confirmed by histopathological examination, lowered levels of serum blood urea nitrogen, creatinine and reduced DNA damage. VO also compensated deficits in the renal antioxidant system. VO intervention significantly inhibited DNA damage, clastogenic effects, and cell cycle arrest in the bone marrow cells of mice. Hematological parameters indicated attenuation of cisplatin-induced myelosuppression. Overall, this study provides for the first time that VO has a protective role in the abatement of cisplatin-induced toxicity in mice which may be attributed to its antioxidant activity.

Keywords: Cisplatin; Genotoxicity; Myelosuppression; Nephrotoxicity; Oxidative stress; Vetiver acetate oil.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / adverse effects
  • Antioxidants / pharmacology
  • Blood Urea Nitrogen
  • Bone Marrow Cells / drug effects*
  • Bone Marrow Cells / metabolism
  • Cisplatin / administration & dosage
  • Cisplatin / adverse effects*
  • Creatinine / blood
  • DNA Damage / drug effects
  • Dose-Response Relationship, Drug
  • Glutathione / blood
  • Kidney / drug effects*
  • Kidney / metabolism
  • Lipid Peroxidation / drug effects
  • Male
  • Mice
  • Micronucleus Tests
  • Oxidative Stress / drug effects*
  • Plant Oils / pharmacology*
  • Toxicity Tests, Acute


  • Antineoplastic Agents
  • Antioxidants
  • Plant Oils
  • Creatinine
  • Glutathione
  • Cisplatin