Perforating corneal injury in rat and pentadecapeptide BPC 157

Exp Eye Res. 2015 Jul;136:9-15. doi: 10.1016/j.exer.2015.04.016. Epub 2015 Apr 24.


Based on its healing effects in various tissues, we hypothesized that the stable gastric pentadecapeptide BPC 157 heals corneal ulcerations in rats and effects corneal transparency. We made a penetrant linear 2-mm incision in the paralimbal region of the left cornea at the 5 o'clock position with a 20-gauge MVR incision knife at 45° under an operating microscope. Medication was BPC 157 (2 pg/mL, 2 ng/mL, and 2 μg/mL distilled water, two eye drops/left rat eye) immediately after injury induction and then every 8 h up to 120 h; controls received an equal volume of distilled water. In contrast to the poor healing response in controls, BPC 157 significantly accelerated the healing process in 2 μg and 2 ng BPC 157-treated eyes, starting 24 h after the injury, and the fluorescein and Seidel tests became negative. The epithelial defects were completely healed at 72 h (2 μg BPC 157-treated group) and at 96 h (2 ng BPC 157-treated group) after injury. Aqueous cells were absent at 96 h and 120 h after injury in the 2 μg and 2 ng BPC 157-treated groups, respectively. In conclusion, BPC 157 effects the rapid regaining of corneal transparency. Whereas controls developed new vessels that grew from the limbus to the penetrated area, BPC 157-treated rats generally had no new vessels, and those that did form in the limbus did not make contact with the penetrated area. Thus, BPC 157 eye drops successfully close perforating corneal incisions in rats.

Keywords: BPC 157; Cornea; Healing promotion; Perforating injury; Rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Animals
  • Anti-Ulcer Agents / therapeutic use*
  • Corneal Perforation / drug therapy*
  • Corneal Perforation / pathology
  • Corneal Ulcer / drug therapy
  • Corneal Ulcer / pathology
  • Disease Models, Animal*
  • Fluorescein
  • Fluorescent Dyes
  • Fluorophotometry
  • Male
  • Ophthalmic Solutions
  • Peptide Fragments / therapeutic use*
  • Proteins / therapeutic use*
  • Rats
  • Rats, Wistar
  • Wound Healing / drug effects*


  • Anti-Ulcer Agents
  • Fluorescent Dyes
  • Ophthalmic Solutions
  • Peptide Fragments
  • Proteins
  • BPC 157
  • Fluorescein