G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

Nat Methods. 2015 Jun;12(6):519-22. doi: 10.1038/nmeth.3370. Epub 2015 Apr 27.


The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • DNA / genetics*
  • Genomics / methods*
  • Humans
  • Mice
  • Nucleic Acid Amplification Techniques / methods*
  • RNA, Messenger / genetics*


  • RNA, Messenger
  • DNA