Conjugated Linoleic Acid Supplementation under a High-Fat Diet Modulates Stomach Protein Expression and Intestinal Microbiota in Adult Mice

PLoS One. 2015 Apr 27;10(4):e0125091. doi: 10.1371/journal.pone.0125091. eCollection 2015.


The gastrointestinal tract constitutes a physiological interface integrating nutrient and microbiota-host metabolism. Conjugated linoleic acids (CLA) have been reported to contribute to decreased body weight and fat accretion. The modulation by dietary CLA of stomach proteins related to energy homeostasis or microbiota may be involved, although this has not been previously analysed. This is examined in the present study, which aims to underline the potential mechanisms of CLA which contribute to body weight regulation. Adult mice were fed either a normal fat (NF, 12% kJ content as fat) or a high-fat (HF, 43% kJ content as fat) diet. In the latter case, half of the animals received daily oral supplementation of CLA. Expression and content of stomach proteins and specific bacterial populations from caecum were analysed. CLA supplementation was associated with an increase in stomach protein expression, and exerted a prebiotic action on both Bacteroidetes/Prevotella and Akkermansia muciniphila. However, CLA supplementation was not able to override the negative effects of HF diet on Bifidobacterium spp., which was decreased in both HF and HF+CLA groups. Our data show that CLA are able to modulate stomach protein expression and exert a prebiotic effect on specific gut bacterial species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cecum / microbiology
  • Diet, High-Fat*
  • Dietary Supplements*
  • Gastric Mucosa / chemistry
  • Gastric Mucosa / drug effects
  • Gastric Mucosa / metabolism
  • Gastrointestinal Microbiome / drug effects*
  • Ghrelin / analysis
  • Leptin / analysis
  • Linoleic Acids, Conjugated / pharmacology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Protein Biosynthesis / drug effects*
  • Real-Time Polymerase Chain Reaction
  • Stomach / drug effects*


  • Ghrelin
  • Leptin
  • Linoleic Acids, Conjugated

Grant support

This work was supported by the grant AGL2012-33692 and the EU-funded project (BIOCLAIMS FP7-244995). The authors' group receives financial support from Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición, CIBERobn. Authors belong to the Nutrigenomics-group, awarded as “Group of Excellence” of CAIB and supported by “Direcció General d’Universitats, Recerca i Transferència del Coneixement” of Regional Government (CAIB) and FEDER funds (EU). AC is supported by a PhD fellowship by Conselleria d'Educació, Cultura i Universitats, Govern de les Illes Balears, a project which is cofinanced by the European Social Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.