Impact of GLP-1 analogue on oxidative damage and hepatic regeneration in experimental 70% hepatectomy model

Hepatogastroenterology. 2015 Mar-Apr;62(138):257-60.

Abstract

Background/aims: The purpose of our study is researching into impact of glucagon like peptide 1 (GLP 1) analogue on liver regeneration after major hepatectomy.

Methodology: 24 wistar albino rats were consecutively divided into 3 groups. Group 1: Control (sham) group day 14 (n = 8), Group 2: Liver resection group day 14 (n = 8); 70% Liver resection was performed, Group 3: Study group day 14 (n = 8); Subsequent to performing 70% liver resection, GLP-1 analogue was administered 2 times a day. (10 μgr/70 kg x 2 times). After 14 day, rats were sacrificed. Oxi- dative stress and antioxidant enzymes and mitochon- drial permeability transition, cytochrome-c, Bax, Bcl- 2, caspase-3, caspase-8 and caspase-3 activity were examined.

Results: 70% Liver resection induced oxi- dative stress of liver tissue was ameliorated by GLP-1 induction. Administration of GLP increased Bcl-2 ex- pression. Decreased expression of cytochrome-c was accompanied by a decrease caspase-3, caspase-8, and Bax expression and caspase-3 activity.

Conclusions: Glp 1 induction plays a regenerative role in the major hepatectomy. This effect is dependent on modulation of the antiapoptotic and antioxidative pathways by GLP 1 expression.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Apoptosis / drug effects
  • Biomarkers / metabolism
  • Blotting, Western
  • Caspase 3 / metabolism
  • Glucagon-Like Peptide 1 / analogs & derivatives
  • Glucagon-Like Peptide 1 / pharmacology*
  • Hepatectomy / methods*
  • Incretins / pharmacology*
  • Liver / drug effects*
  • Liver / metabolism
  • Liver / pathology
  • Liver / physiopathology
  • Liver / surgery*
  • Liver Regeneration / drug effects*
  • Male
  • Models, Animal
  • Oxidative Stress / drug effects*
  • Rats, Wistar
  • Time Factors

Substances

  • Antioxidants
  • Biomarkers
  • Incretins
  • Glucagon-Like Peptide 1
  • Casp3 protein, rat
  • Caspase 3