Measuring ribonucleotide incorporation into DNA in vitro and in vivo

Methods Mol Biol. 2015;1300:123-39. doi: 10.1007/978-1-4939-2596-4_9.

Abstract

Ribonucleotides are incorporated into genomes by DNA polymerases, they can be removed, and if not removed, they can have deleterious and beneficial consequences. Here, we describe an assay to quantify stable ribonucleotide incorporation by DNA polymerases in vitro, and an assay to probe for ribonucleotides in each of the two DNA strands of the yeast nuclear genome.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Alkalies / pharmacology
  • Base Sequence
  • Biological Assay / methods*
  • DNA, Fungal / metabolism*
  • Electrophoresis, Agar Gel
  • Genome, Fungal
  • Molecular Sequence Data
  • Ribonucleotides / metabolism*
  • Saccharomyces cerevisiae / metabolism
  • Staining and Labeling

Substances

  • Alkalies
  • DNA, Fungal
  • Ribonucleotides