Role for T cells, IL-2 and IL-6 in the IL-4-dependent in vitro human IgE synthesis

Immunology. 1989 Nov;68(3):300-6.


The role of T cells and monocytes, as well as that of cytokines, such as IL-1, IL-2 and IL-6, on the IL-4-dependent in vitro human IgE synthesis was investigated. Recombinant IL-4, IL-4-containing T-cell clone supernatants and different combinations of recombinant cytokines failed to induce highly purified B cells to synthesize IgE. IL-4-dependent IgE synthesis was restored by addition to purified B cells of either untreated or mitomycin C-treated autologous T lymphocytes. Addition to purified B cells of autologous monocytes did not restore the IgE response, but usually it exerted a potentiating effect on the synthesis of IgE induced by IL-4 in the presence of suboptimal concentrations of T cells. The activity of T cells apparently preceded that of IL-4 and required a physical contact with B cells. The presence in culture of IL-2 also appeared to be necessary for the T-cell and IL-4-dependent IgE synthesis. Even though not essential, IL-6 was able to potentiate IgE synthesis in most experiments, whereas IL-1 did not display any modulatory effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology
  • Cell Communication
  • Cells, Cultured
  • Humans
  • Immunoglobulin E / biosynthesis*
  • Interleukin-2 / pharmacology*
  • Interleukin-4 / pharmacology*
  • Interleukin-6 / pharmacology*
  • Monocytes / physiology
  • Recombinant Proteins / pharmacology
  • T-Lymphocytes / physiology*


  • Interleukin-2
  • Interleukin-6
  • Recombinant Proteins
  • Interleukin-4
  • Immunoglobulin E