Kinamycin biosynthesis employs a conserved pair of oxidases for B-ring contraction

Chem Commun (Camb). 2015 May 25;51(42):8845-8. doi: 10.1039/c5cc01986a.

Abstract

The biosynthesis of diazobenzofluorene kinamycins requires a hitherto uncharacterized B-ring contraction. Via detailed genetic and enzymatic analyses we unambiguously characterized the conserved pairs of oxidases, AlpJ and AlpK homologs, as nature's machinery for benzofluorenone formation, which paves the way for the investigation of the following diazo assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbazoles / chemistry
  • Carbazoles / metabolism
  • Molecular Structure
  • Oxidoreductases / chemistry
  • Oxidoreductases / metabolism*
  • Stereoisomerism

Substances

  • Carbazoles
  • kinamycin D
  • Oxidoreductases