miR-487b, miR-3963 and miR-6412 delay myogenic differentiation in mouse myoblast-derived C2C12 cells

BMC Cell Biol. 2015 Apr 30;16:13. doi: 10.1186/s12860-015-0061-9.


Background: Skeletal muscle differentiation is a multistep, complex pathway in which several important signaling molecules are involved. Recently, microRNAs (miRNAs), endogenous non-coding small RNAs that regulate mRNAs, have been proposed to be involved in skeletal muscle differentiation. In this study, we identified skeletal muscle differentiation-associated miRNAs by comparing miRNA expression profiles between C2C12 cells and Wnt4 over-expressing C2C12 cells (W4-08), which can spontaneously differentiate into myotubes.

Results: We identified miR-206, miR-133a, and miR-133b as up-regulated miRNAs and miR-487b, miR-3963 and miR-6412 as down-regulated miRNAs in differentiating cells. We focused on the down-regulated miRNAs because their functions were largely unknown. Transfection of mimics of these miRNAs into C2C12 cells resulted in significantly reduced expression of myogenic differentiation markers, including troponin T and myosin heavy chain fast type and slow type, but did not affect the expression of the myogenic transcription factors, MyoD and myogenin.

Conclusions: These miRNAs were characterized as new myogenic differentiation-associated miRNAs which may delay late myogenic differentiation or maturation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Line
  • Down-Regulation
  • Mice
  • MicroRNAs / antagonists & inhibitors
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • MyoD Protein / metabolism
  • Myoblasts / cytology
  • Myoblasts / metabolism
  • Myogenin / metabolism
  • Myosin Heavy Chains / metabolism
  • Oligonucleotides, Antisense / metabolism
  • Transfection
  • Troponin T / metabolism
  • Up-Regulation
  • Wnt4 Protein / genetics
  • Wnt4 Protein / metabolism


  • MicroRNAs
  • MyoD Protein
  • Myogenin
  • Oligonucleotides, Antisense
  • Troponin T
  • Wnt4 Protein
  • Myosin Heavy Chains