Abstract
A panel of five monoclonal antibodies (MAbs) to the HA1 molecule of the influenza B virus of the Victorian lineage with high virus-neutralizing activity was developed. For identification of the virus neutralizing epitopes in HA1 escape mutants (EM) of the influenza BIShandong/07/97 and B/Malaysia/2506/04 virus were selected using virus- neutralizing antibodies (MAbs). Three EMs had single, two--double and one--triple amino acid substitutions (AAS) in HA1 (H122N, A202E, K203T, K2031, K203N or A317V). In addition, AAS N197S was detected in three EMs. A correlation of AAS identified with peculiarities of interaction of EMs with Mabs was discussed.
MeSH terms
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Amino Acid Substitution
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Animals
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Antibodies, Monoclonal / biosynthesis
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Antibodies, Monoclonal / immunology*
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Antibodies, Monoclonal / isolation & purification
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Antibodies, Neutralizing / biosynthesis
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Antibodies, Neutralizing / immunology*
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Antibodies, Neutralizing / isolation & purification
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Antibodies, Viral / biosynthesis
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Antibodies, Viral / immunology*
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Antibodies, Viral / isolation & purification
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Antigen-Antibody Complex / analysis
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Antigen-Antibody Complex / chemistry
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Antigen-Antibody Complex / immunology
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Epitopes / chemistry*
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Epitopes / genetics
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Epitopes / immunology
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Hemagglutinin Glycoproteins, Influenza Virus / chemistry*
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / immunology
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Humans
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Hybridomas / immunology
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Immune Evasion
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Influenza B virus / genetics
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Influenza B virus / immunology*
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Influenza, Human / immunology
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Influenza, Human / virology
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Mice
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Mice, Inbred BALB C
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Models, Molecular
Substances
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Antibodies, Monoclonal
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Antibodies, Neutralizing
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Antibodies, Viral
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Antigen-Antibody Complex
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Epitopes
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Hemagglutinin Glycoproteins, Influenza Virus