DNA repair. Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links

Science. 2015 May 1;348(6234):1253671. doi: 10.1126/science.1253671. Epub 2015 Apr 30.


DNA interstrand cross-links (ICLs) block replication fork progression by inhibiting DNA strand separation. Repair of ICLs requires sequential incisions, translesion DNA synthesis, and homologous recombination, but the full set of factors involved in these transactions remains unknown. We devised a technique called chromatin mass spectrometry (CHROMASS) to study protein recruitment dynamics during perturbed DNA replication in Xenopus egg extracts. Using CHROMASS, we systematically monitored protein assembly and disassembly on ICL-containing chromatin. Among numerous prospective DNA repair factors, we identified SLF1 and SLF2, which form a complex with RAD18 and together define a pathway that suppresses genome instability by recruiting the SMC5/6 cohesion complex to DNA lesions. Our study provides a global analysis of an entire DNA repair pathway and reveals the mechanism of SMC5/6 relocalization to damaged DNA in vertebrate cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatin / chemistry
  • Chromatin / metabolism
  • DNA Damage*
  • DNA Repair Enzymes / metabolism*
  • DNA Repair*
  • DNA Replication*
  • DNA-Binding Proteins / metabolism
  • Mass Spectrometry / methods
  • Proteomics / methods
  • RNA-Binding Proteins / metabolism
  • Xenopus


  • Chromatin
  • DNA-Binding Proteins
  • RNA-Binding Proteins
  • DNA Repair Enzymes