The purpose of this study was to investigate the effects of training muscle groups 1 day per week using a split-body routine (SPLIT) vs. 3 days per week using a total-body routine (TOTAL) on muscular adaptations in well-trained men. Subjects were 20 male volunteers (height = 1.76 ± 0.05 m; body mass = 78.0 ± 10.7 kg; age = 23.5 ± 2.9 years) recruited from a university population. Participants were pair matched according to baseline strength and then randomly assigned to 1 of the 2 experimental groups: a SPLIT, where multiple exercises were performed for a specific muscle group in a session with 2-3 muscle groups trained per session (n = 10) or a TOTAL, where 1 exercise was performed per muscle group in a session with all muscle groups trained in each session (n = 10). Subjects were tested pre- and poststudy for 1 repetition maximum strength in the bench press and squat, and muscle thickness (MT) of forearm flexors, forearm extensors, and vastus lateralis. Results showed significantly greater increases in forearm flexor MT for TOTAL compared with SPLIT. No significant differences were noted in maximal strength measures. The findings suggest a potentially superior hypertrophic benefit to higher weekly resistance training frequencies.