Assessment of Benzene Induced Oxidative Impairment in Rat Isolated Pancreatic Islets and Effect on Insulin Secretion

Environ Toxicol Pharmacol. 2015 May;39(3):1161-9. doi: 10.1016/j.etap.2015.04.010. Epub 2015 Apr 20.

Abstract

Benzene (C6H6) is an organic compound used in petrochemicals and numerous other industries. It is abundantly released to our environment as a chemical pollutant causing widespread human exposure. This study mainly focused on benzene induced toxicity on rat pancreatic islets with respect to oxidative damage, insulin secretion and glucokinase (GK) activity. Benzene was dissolved in corn oil and administered orally at doses 200, 400 and 800mg/kg/day, for 4 weeks. In rats, benzene significantly raised the concentration of plasma insulin. Also the effect of benzene on the release of glucose-induced insulin was pronounced in isolated islets. Benzene caused oxidative DNA damage and lipid peroxidation, and also reduced the cell viability and total thiols groups, in the islets of exposed rats. In conclusion, the current study revealed that pancreatic glucose metabolism is susceptible to benzene toxicity and the resultant oxidative stress could lead to functional abnormalities in the pancreas.

Keywords: Benzene; Environmental pollutants; Insulin resistance; Oxidative stress; Pancreatic islets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzene / administration & dosage
  • Benzene / toxicity*
  • Blood Glucose / metabolism
  • Cell Survival / drug effects
  • DNA Damage
  • Glucokinase / metabolism
  • Insulin / blood*
  • Islets of Langerhans / drug effects*
  • Islets of Langerhans / enzymology
  • Lipid Peroxidation
  • Oxidative Stress*
  • Rats
  • Rats, Wistar

Substances

  • Blood Glucose
  • Insulin
  • Glucokinase
  • Benzene