Strategies for improving the physiological relevance of human engineered tissues

Trends Biotechnol. 2015 Jul;33(7):401-7. doi: 10.1016/j.tibtech.2015.04.003. Epub 2015 Apr 30.


This review examines important robust methods for sustained, steady-state, in vitro culture. To achieve 'physiologically relevant' tissues in vitro additional complexity must be introduced to provide suitable transport, cell signaling, and matrix support for cells in 3D environments to achieve stable readouts of tissue function. Most tissue engineering systems draw conclusions on tissue functions such as responses to toxins, nutrition, or drugs based on short-term outcomes with in vitro cultures (2-14 days). However, short-term cultures limit insight with physiological relevance because the cells and tissues have not reached a steady-state.

Keywords: 2D culture; 3D culture; bioreactors; long-term culture; microfluidics; tissue engineering.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Bioreactors*
  • Cell Culture Techniques*
  • Humans
  • Mice
  • Tissue Engineering*