MALDI-TOF mass spectrometry for early identification of bacteria grown in blood culture bottles

Jean-Benoît Zabbe; Laura Zanardo; Francis Mégraud; Emilie Bessède

doi:10.1016/j.mimet.2015.04.009

MALDI-TOF mass spectrometry for early identification of bacteria grown in blood culture bottles

J Microbiol Methods. 2015 Aug:115:45-6. doi: 10.1016/j.mimet.2015.04.009. Epub 2015 May 2.

Authors

Jean-Benoît Zabbe¹, Laura Zanardo¹, Francis Mégraud¹, Emilie Bessède¹

Affiliation

¹ Laboratory of Bacteriology, University Hospital of Bordeaux, France.

PMID: 25940929
DOI: 10.1016/j.mimet.2015.04.009

Abstract

This note reports an interesting way to rapidly identify bacteria grown from blood culture bottles. Chocolate agar plates were inoculated with 1 drop of the positive blood bottle medium. After a 3-hour incubation, the growth veil was submitted to MALDI-TOF mass spectrometry: 77% of the bacteria present have been correctly identified.

Keywords: Bacteremia diagnosis; Rapid direct identification; Sepsityper alternative; Septic patients.

Publication types

Evaluation Study

MeSH terms

Bacteria / classification
Bacteria / growth & development
Bacteria / isolation & purification*
Bacterial Typing Techniques / methods*
Blood / microbiology
Culture Media / metabolism
Humans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Tandem Mass Spectrometry / methods*

Substances

Culture Media