Relationship Between Ebola Virus Real-Time Quantitative Polymerase Chain Reaction-Based Threshold Cycle Value and Virus Isolation From Human Plasma

J Infect Dis. 2015 Oct 1;212 Suppl 2(Suppl 2):S346-9. doi: 10.1093/infdis/jiv187. Epub 2015 May 3.


We performed a longitudinal analysis of plasma samples obtained from 4 patients with Ebola virus (EBOV) disease (EVD) to determine the relationship between the real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)-based threshold cycle (Ct) value and the presence of infectious EBOV. EBOV was not isolated from plasma samples with a Ct value of >35.5 or >12 days after onset of symptoms. EBOV was not isolated from plasma samples in which anti-EBOV nucleoprotein immunoglobulin G was detected. These data demonstrate the utility of interpreting qRT-PCR results in the context of the course of EBOV infection and associated serological responses for patient-management decisions.

Keywords: Ebola; qRT-PCR; virus isolation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Chlorocebus aethiops
  • Ebolavirus / genetics*
  • Ebolavirus / immunology
  • Hemorrhagic Fever, Ebola / immunology
  • Hemorrhagic Fever, Ebola / virology*
  • Humans
  • Immunoglobulin G / immunology
  • Longitudinal Studies
  • Nucleoproteins / immunology
  • Plasma / immunology
  • Plasma / virology*
  • Real-Time Polymerase Chain Reaction / methods
  • Vero Cells


  • Immunoglobulin G
  • Nucleoproteins