Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP

Acta Crystallogr F Struct Biol Commun. 2015 May;71(Pt 5):609-14. doi: 10.1107/S2053230X15006883. Epub 2015 Apr 21.


The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.

Keywords: Chagas disease; SSGCID; infectious diseases; leishmaniasis; prostaglandin.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Crystallization
  • Humans
  • Hydroxyprostaglandin Dehydrogenases / chemistry*
  • Hydroxyprostaglandin Dehydrogenases / genetics
  • Leishmania major / chemistry*
  • Leishmania major / genetics
  • Molecular Sequence Data
  • NADP / chemistry*
  • NADP / genetics
  • Protein Structure, Secondary
  • Trypanosoma cruzi / chemistry*
  • Trypanosoma cruzi / genetics


  • NADP
  • Hydroxyprostaglandin Dehydrogenases
  • prostaglandin-F synthase

Associated data

  • PDB/4F40
  • PDB/4FZI
  • PDB/4G5D
  • PDB/4GIE