Biofilm production by multiresistant Corynebacterium striatum associated with nosocomial outbreak

Mem Inst Oswaldo Cruz. 2015 Apr;110(2):242-8. doi: 10.1590/0074-02760140373.

Abstract

Corynebacterium striatum is a potentially pathogenic microorganism that causes nosocomial outbreaks. However, little is known about its virulence factors that may contribute to healthcare-associated infections (HAIs). We investigated the biofilm production on abiotic surfaces of multidrug-resistant (MDR) and multidrug-susceptible (MDS) strains of C. striatum of pulsed-field gel electrophoresis types I-MDR, II-MDR, III-MDS and IV-MDS isolated during a nosocomial outbreak in Rio de Janeiro, Brazil. The results showed that C. striatum was able to adhere to hydrophilic and hydrophobic abiotic surfaces. The C. striatum 1987/I-MDR strain, predominantly isolated from patients undergoing endotracheal intubation procedures, showed the greatest ability to adhere to all surfaces. C. striatum bound fibrinogen to its surface, which contributed to biofilm formation. Scanning electron microscopy showed the production of mature biofilms on polyurethane catheters by all pulsotypes. In conclusion, biofilm production may contribute to the establishment of HAIs caused by C. striatum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Adhesion / physiology
  • Biofilms / drug effects*
  • Brazil / epidemiology
  • Catheter-Related Infections / epidemiology
  • Catheter-Related Infections / microbiology*
  • Corynebacterium / classification
  • Corynebacterium / physiology*
  • Corynebacterium / ultrastructure
  • Corynebacterium Infections / epidemiology
  • Corynebacterium Infections / microbiology*
  • Cross Infection / epidemiology
  • Cross Infection / microbiology*
  • Disease Outbreaks*
  • Drug Resistance, Multiple, Bacterial*
  • Electrophoresis, Gel, Pulsed-Field
  • Equipment and Supplies
  • Female
  • Fibrinogen / pharmacology
  • Glass
  • Humans
  • Male
  • Microscopy, Electron, Scanning
  • Middle Aged
  • Polyurethanes
  • Virulence Factors

Substances

  • Anti-Bacterial Agents
  • Polyurethanes
  • Virulence Factors
  • Fibrinogen

Grant support

Financial support: CAPES (E26/101.937/2009), FAPERJ (E26/110.735/2012), CNPq (300236/2010-7), SR-2/UERJ, PNPD-CAPES/MEC (0370088)